Journal: Applied and Environmental Microbiology

Article Title: Spatiotemporal Distribution of the Environmental Microbiota in Food Processing Plants as Impacted by Cleaning and Sanitizing Procedures: the Case of Slaughterhouses and Gaseous Ozone

doi: 10.1128/AEM.01861-20

Figure Lengend Snippet: Average C and T scores for oligotype occurrence matrices generated from the 10 selected core OTUs

Article Snippet: The remaining 5 ml was added to 45 ml of BPW and used to perform microbiological analyses by counting TVCs of mesophilic bacteria on plate count agar (Biolife s.p.a., Milan, Italy) incubated at 30°C for 72 h, Brochothrix spp. on streptomycin-thallous acetate-actidione medium (Biolife) incubated at 25°C for 48 h, Pseudomonas spp. on Pseudomonas agar base with cetrimide-fucidin-cephaloridine selective supplement (Biolife) incubated at 25°C for 48 h, and Staphylococcaceae on mannitol salt agar (Oxoid) incubated at 30°C for 48 h. Microbiological data were expressed and statistically analyzed as log-transformed CFU per square decimeter.

Techniques: Generated

Journal: Applied and Environmental Microbiology

Article Title: Spatiotemporal Distribution of the Environmental Microbiota in Food Processing Plants as Impacted by Cleaning and Sanitizing Procedures: the Case of Slaughterhouses and Gaseous Ozone

doi: 10.1128/AEM.01861-20

Figure Lengend Snippet: Co-occurrence networks of bacterial oligotypes belonging to Achromobacter, Acinetobacter, Psychrobacter, Pseudomonas, Propionibacterium, Carnobacterium, Streptococcus, and Staphylococcus. Each network is based on the log-transformed relative abundance matrix of each genus, and the edges represent significant positive correlations (co-occurrence) between the oligotypes (nodes) by means of Spearman’s correlations (rho > 0.6; FDR, P < 0.001). Nodes were made proportional to the weighted degree (total occurrence of an oligotype in the whole data set) and are colored in relation to the species of belonging. Edges were made proportional to the Spearman’s rho value. The M and D values shown in the box represent the modularity (clustering coefficient) and density (calculated using the ratio of the number of edges) of each genus-based network.

Article Snippet: The remaining 5 ml was added to 45 ml of BPW and used to perform microbiological analyses by counting TVCs of mesophilic bacteria on plate count agar (Biolife s.p.a., Milan, Italy) incubated at 30°C for 72 h, Brochothrix spp. on streptomycin-thallous acetate-actidione medium (Biolife) incubated at 25°C for 48 h, Pseudomonas spp. on Pseudomonas agar base with cetrimide-fucidin-cephaloridine selective supplement (Biolife) incubated at 25°C for 48 h, and Staphylococcaceae on mannitol salt agar (Oxoid) incubated at 30°C for 48 h. Microbiological data were expressed and statistically analyzed as log-transformed CFU per square decimeter.

Techniques: Transformation Assay

Journal: Applied and Environmental Microbiology

Article Title: Spatiotemporal Distribution of the Environmental Microbiota in Food Processing Plants as Impacted by Cleaning and Sanitizing Procedures: the Case of Slaughterhouses and Gaseous Ozone

doi: 10.1128/AEM.01861-20

Figure Lengend Snippet: (a) Pseudo-heatmap summarizing the abundance variations of the 10 core OTUs (>50% of the total abundance in >80% of the samples) occurred during the ozone treatments. Asterisks highlight significant decreases in relative abundances in each environment after ozone treatment at 40, 20, or 4 ppm (FDR-adjusted P value from Wilcoxon’s test). *, 0.05; **, 0.01; ***, 0.001. (b) Viable counts of Brochothrix, Pseudomonas, and Staphylococcus before (ACS) and after (AOT 20) the 20-ppm ozonation carried out in the environment B-PR; box plot colors highlight significant differences between ACS and AOT 20 counts (P < 0.05 [FDR adjusted], Wilcoxon’s test).

Article Snippet: The remaining 5 ml was added to 45 ml of BPW and used to perform microbiological analyses by counting TVCs of mesophilic bacteria on plate count agar (Biolife s.p.a., Milan, Italy) incubated at 30°C for 72 h, Brochothrix spp. on streptomycin-thallous acetate-actidione medium (Biolife) incubated at 25°C for 48 h, Pseudomonas spp. on Pseudomonas agar base with cetrimide-fucidin-cephaloridine selective supplement (Biolife) incubated at 25°C for 48 h, and Staphylococcaceae on mannitol salt agar (Oxoid) incubated at 30°C for 48 h. Microbiological data were expressed and statistically analyzed as log-transformed CFU per square decimeter.

Techniques: